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Rubisco activity in leaves with different FBPase activities, in light and darkness. Freeze-clamped leaf discs from plants expressing 10%, 13%, or normal (100%) levels of FBPase activity were used to determine the activation state of Rubisco before dawn (filled bars) (A), or at midday (open bars, B; i, t, and m, as defined in the text). The stated carboxylase activity of Rubisco (CO2 uptake) represents the mean ± SD from three independent determinations.
