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. 2025 Aug 13;372:fnaf084. doi: 10.1093/femsle/fnaf084

Table 2.

Top performing GLMMs that partitioned variance in the composition of the bacteriome among different hypothetical groupings of the Site 1 and Site 2 filtration regimens.

Fixed effects Random effects Hypothesis
Offset(log(sum)), mosquito-stage, treatment2, mosquito-stage*treatment2 ASV, mesocosm, ASV*mosquito-stage, ASV*mesocosm, ASV*treatment, ASV*mosquito-stage*treatment2 Mosquito bacteriome assembly is modulated by the inclusion of large microbial cells in the inoculate. Treatment2 groups filtrations into individual groups: lab, 0.1 μm, 10 μm, and 30–50 μm.
Offset(log(sum)), mosquito-stage, BacSource3, mosquito-stage*BacSource3 ASV, mesocosm, ASV*BacSource3, ASV*mesocosm, ASV*BacSource3, ASV*mosquito-stage*BacSource3 Mosquito bacteriome assembly is not modulated by the inclusion of large microbial cells in the environment but is modulated by bacteria source (natural environment versus lab environment).
Offset(log(sum)), mosquito-stage, treatment, mosquito-stage*treatment ASV, mesocosm, ASV*mosquito-stage, ASV*mesocosm, ASV*treatment, ASV*mosquito-stage*treatment Mosquito bacteriome assembly is modulated by the inclusion of larger microbial cells in the inoculate. Treatment groups filtrations into individual modules: lab (replicate 1), lab (replicate 2), lab (replicate 3), 0.1 μm, 10 μm, and 30–50 μm.