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(a) UV–vis absorption and emission spectra of 11 μM 1 2+ in an aqueous solution. (b) TrA of 1 2+ in D2O upon 400 nm excitation. TRIR difference absorbance spectra of 0.8 mM 1 2+ in 50 mM phosphate, pH 7, in (c) D2O and (d) H2O. Note that in (d) the spectra were vertically translated to account for the baseline offset (λexc = 400 nm, 2 kHz, 150 fs).
