Skip to main content
. Author manuscript; available in PMC: 2026 Jul 18.
Published in final edited form as: Nat Chem. 2025 Jul 18;17(9):1371–1382. doi: 10.1038/s41557-025-01871-3

Extended Data Figure 4. Dual N- and C-terminal labeling of GS-MBP-TeCH using eSrtA and MccB/subtiligase.

Extended Data Figure 4.

(A) Scheme for dual N- and C-terminal label of GS-MBP-TeCH with MccB/subtiligase and eSrtA. The magenta circle represent azidoAla and the cyan circle represents 5-FAM. (B) Telescoping one-pot dual labeling of GS-MBP-TeCH with eSrtA and MccB/subtiligase. (C) Concurrent one-pot dual labeling of GS-MBP-TeCH with eSrtA and MccB/subtiligase.