Skip to main content
. Author manuscript; available in PMC: 2026 Jul 18.
Published in final edited form as: Nat Chem. 2025 Jul 18;17(9):1371–1382. doi: 10.1038/s41557-025-01871-3

Extended Data Figure 1. MccB-generated thioesters undergo transthioesterification, S-to-N acyl transfer, and native chemical ligation.

Extended Data Figure 1.

(A) MccA-N7G thioester can undergo transthioesterification with MPAA, a thiol nucleophile that cannot directly capture Mcc-N7G-O-AMP. (B) MccA-N7G-O-AMP can undergo thioesterification and S-to-N acyl shift with Cys to form a peptide bond. (C) In the presence of ATP and Mesna, MccB catalyzes native chemical ligation between an unactivated peptide and an N-terminal Cys peptide.