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. Author manuscript; available in PMC: 2026 Jul 18.
Published in final edited form as: Nat Chem. 2025 Jul 18;17(9):1371–1382. doi: 10.1038/s41557-025-01871-3

Extended Data Figure 2. MccB homologs from L. johnsonii and H. pylori can be used in combination with subtiligase for enzyme-catalyzed expressed protein ligation.

Extended Data Figure 2.

(A) LjMccB- and subtiligase-catalyzed ATP-dependent peptide ligation of Ala-Phe (left) or AFAGAGS-azAla (right) to GFP-LjTeCH. (B) HpMccB- and subtiligase-catalyzed ATP-dependent peptide ligation of Ala-Phe (left) or AFAGAGS-azAla (right) to GFP-HpTeCH.