Skip to main content
. Author manuscript; available in PMC: 2025 Aug 25.
Published in final edited form as: Methods Mol Biol. 2019;1914:71–98. doi: 10.1007/978-1-4939-8997-3_5

Table 1.

Working and stock solutions of reagents

Supplements Working solution Stock solution and storage
β-glycerophosphate 10 mM 100 mM in MEMα. Aliquots stored at −20 °C.
L-ascorbic acid 50 μg/mL 1 mg/mL in MEMα. Aliquots stored at −20 °C
Dexamethasone 1–100 nM 10 μM in absolute ethanol. Aliquots stored −80 °C
1,25(OH)2D3 10 nM 10 μM in absolute ethanol. Aliquots stored −80 °C
PGE2 1 μM 10 mM in absolute ethanol. Aliquots stored −80 °C
Insulin 1–10 μg/mL Supplied as 10 mg/mL. Store at 4 °C
Indomethacin 10–50 μM 100 mM in DMSO. Protect from light. Aliquots stored at −70 °C
IBMX 0.25–0.5 mM 250 mM in DMSO. Aliquots stored at −20 °C
Troglitazone 1–10 μM 10 mM in DMSO Aliquot, store at −20 °C
M-CSF 20–50 ng/mL Reconstitute 50 μg/mL in 0.1% BSA (PBS). Aliquots stored at −80 °C
RANKL 10–120 ng/mL Reconstitute 100 μg/mL in 0.1% BSA (PBS). Aliquots stored at −80 °C