Figure 2: Diastolic dysfunction in male and female BPH/2J mice.

Representative images of pulse wave doppler in the LV mitral valve (A) in male and female BPN/3J and BPH2J mice. Bar graphs in B and C represent E/A ratio in male and female mice, respectively. Isovolumic contraction time is displayed in D and E. The LV myocardial performance index values are presented in F and G. Representative Western blotting membranes (H, upper: males; bottom: females) and bar graph quantification of cardiac troponin I normalized by GAPDH (I and J). Representative membranes (upper) and bar graphs of circulating levels of troponin I normalized by the Ponceau red staining in male (K) and female (L) mice. Data are presented as mean ± SEM. *different from BPN/3J 6-week-old; #different from BPN/3J 1.5-year-old; **different from BPH/2J 6-week-old (p<0.05).