Table 6.
DNA protection assay results
| Sample | Lane number | DNA integrity | Description | DNA damage (%) | Protective effect (%) | Inhibition of ROS formation (%) |
|---|---|---|---|---|---|---|
| Control (Native DNA) | 1 | Intact, supercoiled | Native, undamaged pBR322 DNA | 0 | – | – |
| H2O2 (Positive Control) | 3 | Compromised, open circular | H2O2-induced damage | 90 | – | – |
| Essential Oil | 7 | Protected, supercoiled | Protective influence against H2O2-induced damage | 15 | 83 | 68 |
| Methanol Extract | 4 | Protected, supercoiled | Protective effect against H2O2-induced damage | 20 | 78 | 62 |
| Ethyl Acetate Fraction | 6 | Protected, supercoiled | Shielding effect against oxidative stress | 25 | 72 | 65 |
| n-Butanol Fraction | 5 | Protected, supercoiled | Shielding effect against oxidative stress | 30 | 67 | 57 |
| Chloroform fraction | 8 | Partially degraded | Mild protection with limited antioxidant response | 50 | 44 | 35.6 |
| n-Hexane | 9 | Severely degraded | Minimal protection, weak antioxidant response | 70 | 22 | 22.1 |