Table 2.

Regression and validation data for determining RDV and MFX using the proposed methods

Parameters	RDV									MFX
	1DD	RD	MC	AUC		Q-analysis		Bivariate		1DD	RD	MC	AUC		Q-analysis		Bivariate
Wavelength (nm)	250	247–262	247	243–284	290–300	229	245	245	295	290	299–313	299	243–284	290–300	229	245	245	295
Linearity range (µg/mL)		1–15								1–10
Slope	0.0771	0.4736	0.4556	0.348	0.058	0.071	0.028	0.021	0.071	0.1868	1.6476	2.0704	1.150	0.120	0.029	0.021	0.021	0.122
Intercept	0.0093	0.0470	0.0487	0.037	0.006	0.009	0.005	0.005	0.009	0.0083	0.2893	0.2893	0.066	0.005	0.001	0.005	0.005	0.002
LOD (µg/mL)	0.264	0.311	0.302	0.312	0.316	0.301	0.911	0.278	0.314	0.242	0.294	0.262	0.307	0.305	0.278	0.844	0.287	0.259
LOQ (µg/mL)	0.8005	0.941	0.916	0.946	0.957	0.273	0.828	0.844	0.951	0.734	0.892	0.793	0.931	0.925	0.287	0.868	0.868	0.785
Coefficient of determination (r2)	0.9997	0.9996	0.9996	0.9997	0.9995	0.9997	0.9995	0.9997	0.9996	0.9997	0.9995	0.9995	0.9996	0.9995	0.9997	0.9996	0.9996	0.9996
Accuracy (% R) a	98.70	100.09	98.59	100.25		99.84		99.12		101.25	99.91	101.05	98.76		101.07		99.41
Precision (% RSD) b - Repeatability - Intermediate precision	0.522 1.038	1.617 0.869	0.972 0.579	1.233 1.417		1.161 1.47		0.833 1.096		0.635 1.238	1.304 1.465	0.969 1.094	0.742 0.959		0.947 1.328		1.225 1.161

^a Average of 9 determinations (3 concentrations repeated 3 times). ^b %RSD of 9 determinations (3 concentrations repeated 3 times)