Table 7.

Determination of RDV and MFX in spiked human plasma by the proposed methods

Method	RDV			MFX
	Added (µg/mL)	Found* (µg/mL)	%Recovery	Added (µg/mL)	Found* (µg/mL)	%Recovery
Ratio derivative	1	0.956	95.59	1	0.925	92.45
	1	0.943	94.29	4	3.740	93.50
	2	1.877	93.84	8	7.621	95.26
	3	2.772	92.39	4	3.714	92.83
	13	12.538	96.45	5	4.757	95.14
	Mean ± %RSD		94.51 ± 1.665	Mean ± %RSD		93.84 ± 1.389
Ratio difference	1	0.952	95.23	1	0.966	96.61
	1	0.920	92.06	4	3.758	93.97
	2	1.891	94.59	8	7.579	94.75
	3	2.740	91.36	4	3.836	95.92
	13	12.426	95.59	5	4.697	93.95
	Mean ± %RSD		93.77 ± 2.055	Mean ± %RSD		95.04 ± 1.251
Mean centering	1	0.931	93.13	1	0.953	95.28
	1	0.920	92.03	4	3.801	95.03
	2	1.827	91.34	8	7.598	94.98
	3	2.823	94.11	4	3.693	92.32
	13	12.141	93.39	5	4.665	93.29
	Mean ± %RSD		92.80 ± 1.192	Mean ± %RSD		94.18 ± 1.385
Area under the curve	1	0.933	93.31	1	0.961	94.66
	1	0.946	94.63	4	3.771	93.98
	2	1.928	96.40	8	7.773	95.86
	3	2.854	95.13	4	2.809	91.52
	13	12.093	93.02	5	3.694	92.62
	Mean ± %RSD		94.50 ± 1.461	Mean ± %RSD		93.73 ± 1.816
Q-analysis method	1	0.948	94.75	1	0.923	92.33
	1	0.961	96.09	4	3.756	93.89
	2	1.898	94.91	8	7.501	93.76
	3	2.856	95.21	4	3.728	93.20
	13	12.268	94.37	5	4.626	92.52
	Mean ± %RSD		95.07 ± 0.683	Mean ± %RSD		93.14 ± 0.759
Bivariate method	1	0.94	93.71	1	0.96	96.03
	1	0.93	92.80	4	3.81	97.04
	2	1.93	94.07	8	7.53	96.40
	3	2.76	92.06	4	3.72	93.04
	13	12.39	95.31	5	4.86	97.26
	Mean ± %RSD		94.06 ± 1.897	Mean ± %RSD		95.12 ± 1.729