Table 4.

Inter- and intra-day accuracy and precision of alectinib quantification in rat plasma

	QC	Nominal concentration (ng/mL)	Measured concentration (ng/mL; mean ± SD)	Accuracy (%)a	Precision (RSD, %)b
Inter-day	LLOQ	5	4.87 ± 0.53	97.4	10.9
	LQC	15	14.3 ± 1.0	95.6	7.46
	MQC	100	95.8 ± 6.9	95.8	7.21
	HQC	800	808 ± 54	102	6.66
Intra-day	LLOQ	5	4.82 ± 0.53	96.0	10.5
	LQC	15	14.7 ± 1.2	98.0	8.36
	MQC	100	97.0 ± 10.5	97.1	10.8
	HQC	800	812 ± 46	101	5.64

^a Accuracy was calculated by measured concentration/nominal concentration × 100%

^b Precision, expressed as the relative standard deviation (RSD), was calculated as the standard deviation of the concentration/mean concentration × 100%