Table 5.

Recovery and matrix effect of alectinib and the internal standard (ponatinib) in rat plasma (mean ± SD, n = 5)

	Alectinib			Internal standard (ponatinib)
	LQC (15 ng/mL)	MQC (100 ng/mL)	HQC (800 ng/mL)	100 ng/mL
Recovery (%)a	105 ± 3	104 ± 7	98.3 ± 5.3	98.6 ± 2.1
Matrix effect (%)b	87.5 ± 9.3	89.6 ± 8.9	86.1 ± 8.2	92.1 ± 4.6

^a Matrix effect (%) = peak area of analyte-added post-precipitation/peak area of analyte in neat analyte solution × 100

^b Recovery (%) = analyte peak area of an analyte added before precipitation/peak area of an analyte added after precipitation × 100