Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

letter

. 2025 Aug 22;14:109. doi: 10.1186/s40164-025-00700-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Triapine stimulates FAS upregulation via ER stress and NFκB. (A) Expression of receptor genes in SW480 cells (1 µM Triapine, 18 h). Values are mean log fold change compared to control. (B) Representative flow cytometry histograms and quantification (mean fluorescence intensities (MFI) ± SD) of FAS expression of Triapine-treated CT-26 cells. (C) Representative IHC images (scale bar: 100 μm) and (D) associated quantification of FAS expression (mean % ± SEM) in CT-26 tumors after treatment with Triapine. (E) Caspase 8 activity and (F) cell viability of HCT116 cells after 24 h Triapine preincubation, followed by 24 h FASL treatment. Values are mean ± SD from one representative experiment out of three, normalized to untreated control. (G) Impact of the ER-stress inhibitor 4-PhB on Triapine-induced NFκB activity was analyzed using NFκB reporter HEK293-Blue™ hTLR7 cells after 24 h. Values are mean ± SD of three independent experiments normalized to control and cell number. Impact of (H) 4-PhB or ± (I) Bay 11-7082 on Triapine-induced FAS expression in HCT116 cells with functional caspase 8 signaling after 24 h treatment. Values are MFI ± SD of three independent experiments, normalized to control. Significance was determined by one-way, two-way ANOVA with Bonferroni´s or Dunnett´s multiple comparisons test or unpaired T-test (**** p < 0.001, *** p < 0.001, ** p < 0.01, * p < 0.05). (J) Scheme summarizing the immune-enhancing activities of Triapine via induction of ICD and FAS upregulation.