Table 1.
Summary of the LAMP-assay results, K-TYPE, MLST and source of the strains
| Strain | K-TYPE y MLST | iucC | iroC | rmpA | rmpA2 | ybt | clb | Conventional PCR | Source | Kleborate score |
|---|---|---|---|---|---|---|---|---|---|---|
| BUH1 | K2-ST66 | 12:11 | 17:11 | 10:22 | 10:00 | 7:43 | 8:48 | iucC, iroC, rmpA, rmpA2, ybt, clb | Bronchial aspirate | 5 |
| BUH2 | K1-ST23 | 10:46 | 10:10 | 10:04 | 9:08 | - (curve) | 7:34 | iucC, iroC, rmpA, rmpA2, ybt, clb | Liver abscess | 5 |
| BUH3 | K1-ST23 | 12:57 | 11.35 | - (curve) | 10:38 | - (curve) | 8:33 | iucC, iroC, rmpA, rmpA2, ybt, clb | Liver abscess | 5 |
| BUH4 | K1-ST23 | 11:53 | 12:16 | 8:53 | 9:52 | 7:18 | 6:58 | iucC, iroC, rmpA, rmpA2, ybt, clb | Liver abscess | 5 |
| BUH6 | K57-ST412 | 12:03 | 11:07 | 11:05 | 8:54 | – | – | iucC, iroC, rmpA y rmpA2 | Bile | 3 |
| K30B1 | K30-ST198 | – | 12:45 | - (curve) | – | 7:48 | – | rmpA, ybta, iroC | Liver abscess | 1 |
| K2-HUMV1 | K2-ST66 | 9:45 | 11:46 | 8:22 | – | 7:03 | 7:58 | iucC, iroC, rmpA, rmpA2, ybt, clb | Blood culture | 5 |
| VHVKP1 | K1-ST23 | 11:39) | 11:06 | 11:08 | 10:33 | 9:11 | 7:04 | iucC, iroC, rmpA, rmpA2, ybt, clb | Urine | 5 |
| VHVKP2 | K1-ST23 | - (curve) | 11:57 | 10:23 | 10:10 | 7:42 | 6:50 | iucC, iroC, rmpA, rmpA2, ybt, clb | Abdominal abscess | 5 |
| VHVKP3 | K2-ST6997 | 10:12 | 9:54 | 7:24 | – | 6:19 | – | iucC, iroC, rmpA, ybt | Surgical wound | 4 |
| VHVKP4 | K1-ST23 | 8:24 | 8:23 | 8:51 | 8:16 | 6:22 | 6:43 | iucC, iroC, rmpA, rmpA2, ybt, clb | Sputum | 5 |
| VHVKP5* | K54-ST714 | 11:57 | 12:38 | - (curve) | 8:59 | 9:02 | – | iucC, iroC, rmpA, rmpA2, ybt | Urine | 4 |
| VHVKP6 | K2-ST380 | - (curve) | 10:31 | 9:30 | – | 9:07 | – | iucC, iroC, rmpA, ybt | Blood culture | 4 |
| VHVKP7 | K2-ST380 | 11:05 | 10:00 | 10:36 | – | 8:53 | – | iucC, iroC, rmpA, ybt | Sputum | 4 |
| VHVKP8 | K1-ST23 | – | 11:50 | – | – | 10:53 | 8:51 | iroC, ybt, clb | Urine | 2 |
| VHVKP9 | K2-ST65 | - (curve) | 13:18 | 13:05 | 10:10 | – | – | iucC, iroC, rmpA, rmpA2 | Blood culture | 3 |
| VHVKP10 | K62-ST45 | 7:56 | - | - | - | 7:49 | – | iucC, ybt | Blood culture | 4 |
| VHVKP11* | K54-ST714 | 12:13 | 11:14 | 9:00 | 9:52 | 8:57 | – | iucC, iroC, rmpA, rmpA2, ybt | Sputum | 4 |
| VHVKP12 (NC) | K25-ST17 | – | – | – | – | 9:59 | – | ybt | Ear | 1 |
| VHVKP13 (NC) | K102-ST307 | – | – | – | – | 8:35 | - | ybt | Urine | 1 |
Additionally, we included the virulence scores of the study strains and the virulence genes proposed by the Kleborate [8], which were detected by conventional PCR. The positive results of the LAMP-based assay are reported as the time to detection, while negative results are indicated as a hyphen (-). Strains VHVKP12 and VHVKP13 were used as negative controls. Although the kit failed to detect eight target genes, a fluorescence curve was observed in all cases. These cases are shown as “- (curve)”. Strains marked with an asterisk ((*) caused two distinct episodes with different clinical presentations, occurring one and a half months apart