Figure 2.

Modulation of caspase-2 and HSP protein levels in hyperthermia-enhanced apoptosis during multimodal treatment. HCT116 cells were treated with 2μM ART for 20h at 37°C, followed by treatment with or without 1 ng/mL rhTRAIL for an additional 4h. In parallel, some samples were exposed to hyperthermia at 42°C for 1, 2, 3, or 4h and then returned to 37°C for the remainder of the incubation period, respectively. Whole-cell extracts were analyzed with an immunoblotting assay using indicated antibodies.