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. Author manuscript; available in PMC: 2025 Aug 25.
Published in final edited form as: Cancer Lett. 2023 Nov 15;580:216493. doi: 10.1016/j.canlet.2023.216493

Figure 2. 2-OG inhibited DNA damage responses produced by chemotherapy.

Figure 2.

(A) Relative cell survival rate was determined in H1 cells treated with 0, 25, or 50 μM 2-OG in the presence of 1.25μM Dox for 2 days. **, p<0.01 by ANOVA analysis. (B) The protein expression levels of γ-H2aX and α-tub were examined in H1 CCA cells treated with 2-OG overnight and challenged with 1μM Dox for 2 hours. (C) ASPH, γ-H2aX, and α-tub were determined in 0, 6, 17, 100, 1000 μM 2-OG pre-treated HEK-293T cells expressing empty vector or ASPH for 24 hours before receiving DMSO control or 20μM CPT challenge for 2 hours. (D) γ-H2aX, GAPDH, and myc-ASPH expression was determined in HEK-293T cells expressing either ASPH (full-length) or ASPH variant form 3 (ASPHv3), which has no catalytic domain or enzymatic activity, in the presence of 4μM GCB plus 10μM Pt or 1μM Dox for 2 hours.