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. Author manuscript; available in PMC: 2025 Aug 25.
Published in final edited form as: Cancer Lett. 2023 Nov 15;580:216493. doi: 10.1016/j.canlet.2023.216493

Figure 3. ASPH expression negatively regulated DDR and inhibited cell death.

Figure 3.

(A) γ-H2aX, α-tub, and ASPH were determined in SSP25 and HuCCT1 treated with shLuc or shASPH in the presence of 2.5μM Dox or 20μM CPT for 2 hours. (B) Immunoblotting results of ASPH, γ-H2aX, and α-tub in OUMS29 and HEK-293T cells expressing empty vector or ASPH and treated with as indicated in the presence of 20μM CPT for 2 hours. (C) The relative comet tail lengths of H1-shLuc, H1-shASPH, (D) SSP25-shLuc, and SSP25-shASPH CCA cells treated with control (Veh), 2.5μM Dox, 10μM Pt plus 1μM GCB for 2 hours were shown. *, p<0.05; **, p<0.01, when compared to the relevant controls.