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. 2002 Mar;68(3):1082–1087. doi: 10.1128/AEM.68.3.1082-1087.2002

FIG. 3.

FIG. 3.

Ethidium-stained agarose gel (1.5%) of RT-PCR products (the entire mRNA of the nir gene) from dilutions of RNA extracted from the pond water sample using the SLS-phenol and the DivoLab-phenol methods [(a) and (b) in the lane descriptions refer to duplicate extracted filters]. Lanes 1 and 13, DNA ladders; lane 2, control without RT step; lane 3, control without template; lane 4, SLS-phenol undiluted (a); lane 5, SLS-phenol undiluted (b); lane 6, DivoLab-phenol undiluted (a); lane 7, DivoLab-phenol undiluted (b); lane 8, SLS-phenol diluted 1:10 (a); lane 9, SLS-phenol diluted 1:10 (b); lane 10, DivoLab-phenol diluted 1:10 (a); lane 11, DivoLab-phenol diluted 1:10 (b); lane 12, DivoLab-phenol diluted 1:100.