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. 2002 Mar;68(3):1082–1087. doi: 10.1128/AEM.68.3.1082-1087.2002

FIG. 4.

FIG. 4.

SSCP patterns obtained with single-stranded PCR products of 16S rRNA genes (lanes 2 to 9) and single-stranded RT-PCR products (lanes 11 to 17) amplified from pond bacterioplankton extracted by the SLS-phenol and DivoLab-phenol methods [(a) and (b) in the lane descriptions refer to duplicate extracted filters]. Lanes 1, 10, and 18, DNA ladders; lane 2, SLS-phenol undiluted (a); lane 3, SLS-phenol diluted 1:10 (a); lane 4, SLS-phenol undiluted (b); lane 5, SLS-phenol diluted 1:10 (b); lane 6, DivoLab-phenol undiluted (a); lane 7, DivoLab-phenol diluted 1:10 (a); lane 8, DivoLab-phenol undiluted (b); lane 9, DivoLab-phenol diluted 1:10 (b); lane 11, SLS-phenol undiluted (a); lane 12, SLS-phenol diluted 1:10 (a); lane 13, SLS-phenol undiluted (b); lane 14, SLS-phenol diluted 1:10 (b); lane 15, DivoLab-phenol undiluted (a); lane 16, DivoLab-phenol diluted 1:10 (a); lane 17, DivoLab-phenol undiluted (b). Between lanes 17 and 18, a lane with a different marker was excised by using Adobe Photoshop.