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. Author manuscript; available in PMC: 2026 Jul 30.
Published in final edited form as: Structure. 2025 Jul 30;33(10):1717–1727.e4. doi: 10.1016/j.str.2025.07.007

Figure 2. Apoferritin reconstructions imaged at 100 keV.

Figure 2.

(A) Representative motion-corrected and dose-weighted image of apoferritin sample in vitreous ice with 0.7 Å/pix (scale bar – 20 nm).

(B) Three-dimensional reconstruction of apoferritin with octahedral symmetry, highlighting the asymmetric unit in blue.

(C) Examples of high-quality density at 0.55 Å/pix magnification

(D) Examples of high-quality density at 0.7 Å/pix magnification

(E) Examples of high-quality density at 0.9 Å/pix magnification

(F) Gold-standard Fourier Shell Correlation (FSC) curves comparing the two independently refined half-maps of the datasets collected at three different magnifications: 0.55 Å/pix (grey), 0.7 Å/pix (blue), and 0.9 Å/pix (yellow). Resolutions are estimated using the FSC = 0.143 criterion.

(G) Map-model Fourier Shell Correlation (FSC) curves comparing the experimental maps and the calculated model maps of the three datasets: 0.55 Å/pix (grey), 0.7 Å/pix (blue), and 0.9 Å/pix (yellow). Resolutions are estimated using the FSC = 0.5 criterion.

(H) B-factor plots for four different datasets, including three collected at varying magnifications with the Falcon C detector (0.55 Å/pix in grey, 0.7 Å/pix in blue, and 0.9 Å/pix in yellow), and one dataset at a magnification of 0.75 Å/pix collected with Ceta-F (red).