KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, peptides, and recombinant proteins | ||
| Adeno-associated Virus 9 with GFP tag | Recieved from the Thompson’s team at Thermo Fisher Scientific Cell Biology | N/A |
| CAK7 complex (AMPPNP bound) | Received from the Greber laboratory at Institute for Cancer Research, London | N/A |
| Human GABAA receptor β3-subunit with a truncated M3-M4 complexed with Megabody-2521 | Received from Aricescu laboratory at MRC-LMB Cambridge, UK | N/A |
| Lyophilized Aldolase from rabbit muscle | Sigma-Aldrich | A2714 |
| Lyophilized Human haemoglobin | Sigma-Aldrich | H7379 |
| Thermoplasma acidophilum 20S proteasome | Received from Plitzko laboratory at MPI for Biochemistry, Germany | N/A |
| Mouse Apoferritin | Received from the Kikkawa laboratory at Tokyo University | N/A |
| Transthyretin (A2 bound) | Received from the Lander laboratory at The Scripps Research Institute, La Jolla | N/A |
| 0.5M HEPES. pH 7.5 solution, 250ml | Thermo Fisher Scientific | J61275.AK |
| 5M NaCl aq. Solution autoclaved | Thermo Fisher Scientific | J61890.AK |
| AAV-MAX production system | Thermo Fisher Scientific | A51217 |
| Deposited data | ||
| AAV9-GFP at 2.8 Å from Falcon C | This study | EMD-51540 |
| Apoferritin at 2.2 Å from Falcon C imaged at 0.55 Å/pixel | This study | EMD-51463 |
| Apoferritin at 2.1 Å from Falcon C imaged at 0.7 Å/pixel | This study | EMD-51481 |
| Apoferritin at 2.2 Å from Falcon C imaged at 0.9 Å/pixel | This study | EMD-51483 |
| CAK complex at 4 Å from Falcon C | This study | EMD-51519 |
| GABAA receptor at 2.8 Å from Falcon C imaged at 0.7 Å/pixel | This study | EMD-51506 |
| GABAA receptor at 3.2 Å from Falcon C imaged at 0.9 Å/pixel | This study | EMD-51508 |
| Human haemoglobin at 5 Å from Falcon C | This study | EMD-51525 |
| Human transthyretin at 3.5 Å from Falcon C | This study | EMD-51526 |
| Rabbit muscle aldolase at 3 Å from Falcon C imaged at 0.55 Å/pixel | This study | EMD-51511 |
| Rabbit muscle aldolase at 2.8 Å from Falcon C imaged at 0.55 Å/pixel | This study | EMD-51512 |
| T20S proteosome at 2.7 Å from Falcon C imaged at 0.7 Å/pixel | This study | EMD-51487 |
| T20S proteosome at 2.9 Å from Falcon C imaged at 0.9 Å/pixel | This study | EMD-51503 |
| Apoferritin at 2.6Å from Ceta-F | This study | EMD-13769, EMPIAR-10844 |
| GABAA receptor at 3.4 Å from Ceta-F | This study | EMD-13816, EMPIAR-10858 |
| T20S proteosome at 3.0 Å from Ceta-F | This study | EMD-14249, EMPIAR-10961 |
| Software and algorithms | ||
| Chimera r1.17 | Pettersen et al. [32] | https://www.cgl.ucsf.edu/chimera/ |
| ChimeraX r1.6 | Pettersen et al. [32] | https://www.cgl.ucsf.edu/chimerax/ |
| cryoSPARC (v4.x) | Punjani et al. [30] | https://cryosparc.com/ |
| Phenix | Liebschner et al. | https://phenix-online.org/ |
| pyEM | Asarnow et al.[33] | https://github.com/asarnow/pyem |
| Relion 4.x | Zivanov et al. [20] | https://github.com/3dem/relion |
| Thermo Scientific Smart EPU Software | Thermo Fisher Scientific | https://www.thermofisher.com/smart-epu.html |