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. 2025 Aug 22;11(34):eadw4289. doi: 10.1126/sciadv.adw4289

Fig. 2. Cyclic voltammetry measurements of dried and live ANME/SRB consortia.

Fig. 2.

(A) Live biomass was pressed onto the IDA using a Gelrite plug in a syringe to promote physical attachment and electrochemistry measurements under anoxic conditions in PBS solution (pH 7.4). A platinum wire served as the counter electrode and Ag/AgCl (1 M KCl) as the reference electrode. (B) Representative cyclic voltammograms of dry-deposited and live ANME-1/Desulfofervidus consortia show clear redox signals, whereas PBS and Gelrite backgrounds show no redox signal. (C) Representative cyclic voltammograms show redox peaks in diverse ANME/SRB consortia after subtraction of PBS background current from the same IDAs. (D) Comparison of peak current heights for ANME-1/Desulfofervidus consortia subjected to increasing scan rates, revealing a linear dependence on the square root of scan rates and indicating that electron transport through the consortia is rate limiting.