Fig. 3. Generator-collector measurements of ANME/SRB consortia.
(A and B) Schematics of expected outcomes for generator-collector measurements: (A) a blank background lacking conductive material and (B) ANME/SRB consortia containing redox conductive cytochromes that mediate electron transport across the 5-μm gap. The generator electrode sweeps to a more negative potential, reducing the redox component, while the collector electrode is held at a constant positive potential, reoxidizing it. (C and D) Current measured at the generator electrode sweeping from +335 to −265 mV for (C) backgrounds and (D) live ANME/SRB consortia. (E and F) Currents measured at the collector electrodes holding at a constant +335 mV for (E) backgrounds and (F) live ANME/SRB consortia. Together, the generator-collector measurements reveal that the redox-active components, consistent with multiheme cytochrome c, in diverse ANME/SRB consortia are capable of facilitating micrometer-scale electron transport from generator electrodes to collector electrodes.
