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. 2025 Aug 22;11(34):eadx9763. doi: 10.1126/sciadv.adx9763

Fig. 6. Twist1- and Runx2-positive cells are not affected after miR-200a treatment in Twist1+/− mice.

Fig. 6.

(A) Twist1 and Runx2 IF expression in P21 WT mouse sagittal sections of the coronal suture. Twist1 (green staining) and Runx2 (green staining) are expressed in the suture cells and dura mater. (B) Twist1 and Runx2 IF expression in P21 Twist1+/− heterozygous mice, sagittal sections of the coronal suture. (C) Twist1 and Runx2 IF expression in P21 Twist1+/− heterozygous mice treated with EV nanoparticles, sagittal sections of the coronal suture. (D) Twist1 and Runx2 IF expression in P21 Twist1+/− heterozygous mice treated with miR-200a nanoparticles, sagittal sections of the coronal suture. (E and F) Quantitation of Twist1 (n = 3, three mice were analyzed for Twist1 expression) and Runx2 (n = 4, four mice were analyzed for Runx2 expression) expression levels in the mouse sagittal sections. ****P < 0.0001; n.s., not statistically significant.