Skip to main content
. 2025 Aug 22;11(34):eadx9763. doi: 10.1126/sciadv.adx9763

Fig. 7. Gli1- and Six2-positive cells are regulated by miR-200a treatment in Twist1+/− mice.

Fig. 7.

(A) Gli1 and Six2 IF expression in P21 WT mouse sagittal sections of the coronal suture. Gli1 (red staining) and Six2 (green staining) are expressed in suture cells and dura mater. (B) Gli1 and Six2 IF expression in P21 Twist1+/− heterozygous mice, sagittal sections of the coronal suture. (C) Gli1 and Six2 IF expression in P21 Twist1+/− heterozygous mice treated with EV nanoparticles, sagittal sections of the coronal suture. (D) Gli1 and Six2 IF expression in P21 Twist1+/− heterozygous mice treated with miR-200a nanoparticles, sagittal sections of the coronal suture. (E and F) Quantitation of Gli1 and Six2 expression levels in the mouse sagittal sections (n = 3, three mice were analyzed for Gli1 and Six2 expression). ****P < 0.0001.