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. 2025 Aug 22;11(34):eadx9763. doi: 10.1126/sciadv.adx9763

Fig. 8. Wnt10b- and β-catenin–positive cells are increased in the Twist1+/− coronal sutures and decreased after miR-200a treatment.

Fig. 8.

(A) Wnt10b and β-catenin IF expression in P21 WT mouse sagittal sections of the coronal suture. Wnt10b (green staining) and β-catenin (red staining) are expressed in suture cells. (B) Wnt10b and β-catenin IF expression in P21 Twist1+/− heterozygous mice, sagittal sections of the coronal suture. (C) Wnt10b and β-catenin IF expression in P21 Twist1+/− heterozygous mice treated with EV nanoparticles, sagittal sections of the coronal suture. (D) Wnt10b and β-catenin IF expression in P21 Twist1+/− heterozygous mice treated with miR-200a nanoparticles, sagittal sections of the coronal suture. (E and F) Quantitation of Wnt10b and β-catenin expression levels in the mouse sagittal sections (n = 3, three mice were analyzed for Wnt10b and β-catenin expression). *P < 0.05.