Fig. 3. BAY-3827 and BAY-974 kinetics.

(A to C) Inhibition of AMPK complexes in cell-free assays by BAY-3827 in high (200 μM) and low (20 μM) ATP concentrations and by (D) BAY-974 at 200 μM ATP. Data are n = 3 represented as means ± SEM. (E) IC₅₀ values (nM) of BAY-3827 inhibition of AMPK complexes in different ATP conditions. (F and G) HTRF assays in U2OS cells measuring phosphorylation (%) of Ser⁷⁹-ACC1 following BAY-3827 or BAY-974 treatment and phosphorylation (%) of (H and I) Thr¹⁷²-AMPKα in the presence or absence of AMPK activator MK-8722 (10 μM). (J) Estimated IC₅₀ and EC₅₀ values (μM) shown, respectively, from (F) to (I). Data are means ± SEM with n = 3 technical replicates from three independent experiments.