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. 2025 Aug 22;11(34):eadt9645. doi: 10.1126/sciadv.adt9645

Fig. 3. P2Y10 is highly expressed on ILCs and mediates intestinal residency of ILCs.

Fig. 3.

(A) Transcriptome analysis of sorted intestinal ILCs (Lin−CD127+Thy1.2+) in Ctrl and Abx-treated mice. Highly expressed genes in Ctrl ILCs were performed GO enrichment analysis. (B) Expression of GPCRs was shown, and top 10 of the most specifically and highly expressed GPCRs in Ctrl ILCs were marked. (C) Relative mRNA levels of the candidate GPCRs in sorted Ctrl and Abx ILCs. Fold changes were normalized to Actb. (D) Immunofluorescence of P2Y10-expressing intestinal ILCs (white arrows). Scale bars, 30 μm. (E to G) Flow cytometry analysis of ILC1s, ILC2s, and ILC3s in the SI (E), mLN (F), and spleen (G) from P2ry10+/+ and P2ry10−/− mice. n = 5. (H) Immunofluorescence of the SI from P2ry10+/+ and P2ry10−/− mice for analysis of gut-associated lymphoid tissues. Scale bars, 2000 μm (left), 70 μm (right). n = 5. (I) Flow cytometry analysis of CD69+ and S1PR1+ ILCs derived from SI of P2ry10+/+ and P2ry10−/− mice. n = 5. (J) P2ry10+/+ or P2ry10−/− CD45.2+ donor mice underwent parabiosis surgery with CD45.1+ recipient mice. Flow cytometry analysis of donor-derived intestinal ILC1s, ILC2s, and ILC3s from the CD45.1+ parabionts. n = 4. (K) Relative mRNA levels of P2RY10 in ILCs from noninflamed and inflamed intestinal samples of patients with CD. Fold changes were normalized to ACTB. n = 4 independent experiments. (L) Flow cytometry analysis of the expression of P2Y10 in intestinal ILCs from patients with CD. n = 6. Data are representative of at least three independent experiments and are shown as the means ± SD. Statistical analysis was performed by using unpaired two-tailed Student’s t test (*P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant). SSC, side scatter; W, width.