Table 2. Qualitative inter-laboratory analysis of an anonymised panel of influenza samples using the JRC-HA and JRC-MP digital PCR assays (n = 40).
| Subtype | Pathotype | Lineage/clade | HA IZSVe + | HA JRC + | % HA agreement | MP IZSVe + | MP JRC + | % MP agreement |
|---|---|---|---|---|---|---|---|---|
| H1N1 | LPAI | Avian | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H1N1 | LPAI | Swine 1A.3.3.2 [37] | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H3N2 | LPAI | Swine | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H3N8 | LPAI | Eurasian | 0/2 | 0/2 | 100 | 2/2 | 2/2 | 100 |
| H5N1 | HPAI | Eurasian, 2.3.4.4b | 26/26 | 26/26 | 100 | 26/26 | 26/26 | 100 |
| H5N1 | LPAI | Eurasian | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H5N2 | LPAI | Eurasian | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H5N3 | LPAI | Eurasian | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H7N7 | HPAI | Eurasian | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H7N7 | LPAI | Eurasian | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| H9N2 | LPAI | Eurasian, clade Y8 [38] | 0/1 | 0/1 | 100 | 1/1 | 1/1 | 100 |
| Allantoic fluid | 0/3 | 0/3 | 100 | 0/3 | 0/3 | 100 | ||
| Total | 40 | 40 | 100 | 40 | 40 | 100 | ||
+: only the positive results are reported; HA: JRC-HA assay ; HPAI: high pathogenic avian influenza virus; IZSVe: Istituto Zooprofilattico Sperimentale delle Venezie; JRC: Joint Research Centre; LPAI: low pathogenic avian influenza virus; MP: JRC-MP assay.
The number of positive samples over the total number of isolates for a given subtype is shown. Unless otherwise specified, all samples were originally isolated from bird species.