FIG. 4.

Immunofluorescence microscopy of hydrophobin Hyd1. Localization of the Hyd1 protein was achieved by immunofluorescence staining using anti-Sc3 antiserum on submerged and aerial hyphae of T. terreum. (A) The hydrophobin was detected at the perimeter of the cell in aerial mycelium. (B) No fluorescence was observed in substrate hyphae. The accumulation of hydrophobin in symbiotic tissue of T. terreum with pine was shown by staining with anti-Sc3 antiserum (C and E) in the hyphal mantle and the Hartig net of compatible mycorrhiza between T. terreum and pine (C to F). (G and H) Host specificity was shown in comparison to an interaction with the non-native host spruce, where hardly any staining was visible for the Hartig net. The mantle showed comparable levels of the protein regardless of compatibility. Bars, 50 μm.