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. 2025 Aug 21;66(11):49. doi: 10.1167/iovs.66.11.49

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Figure 1. — Overview of the Image Processing Pipeline for Optical Coherence Tomography Angiography Analysis. This figure provides an overview of the processing steps used for the quantitative analysis of OCTA images from the superficial capillary plexus (SCP), deep capillary plexus (DCP), and full retina slab. The workflow begins with the averaging of exported images for each slab to enhance vessel visualization and reduce noise. The foveal avascular zone (FAZ) and watermarks are first manually selected and then semi-automatically removed using a Fiji macro, which also calculates various OCTA metrics and saves the processed images for visualization. This integrated approach ensures a reliable assessment of retinal microvascular changes, allowing for a comprehensive evaluation of perfusion characteristics across different retinal layers.