Table 1:

Sequences of forward and reverse oligonucleotides used for phenotypic characterization by RT-qPCR. 18S gene was used as a housekeeping gene to normalize results.

Phenotype marker	Forward	Reverse
P2RY12	5’-GTGATGCCAAACTGGGAACAGG-3’	5’-CTGGTGGTCTTCTGGTAGCGAT-3’
CD68	5’-CTCCAAGCCCAGATTCAGATT-3’	5’-GGGAATGAGAGAAGCAGGTG-3’
CD45	5’-GGTTTCAAAGAACCCAGGAAATAC-3’	5’-ACATCGAGTGACCATGACAATAA-3’
CD86	5’-CCCAGAACCTAAGAAGATGAGTG-3’	5’-GCTCGTAACATCAGGGAATGA-3’
CD32	5’-CTCTGGTCAAGGTCACATTCTT-3’	5’-TTGGATGAGAACAGCGTGTAG-3’
CD206	5’-GGACGTGGCTGTGGATAAAT-3’	5’-ACCCAGAAGACGCATGTAAAG-3’
CD163	5’-GTGTGATGACTCTTGGGACTT-3’	5’-CCTGACCAAACTCTGCTTCT-3’
18S	5’-CCCACGGAATCGAGAAAGAG-3’	5’-TTGACGGAAGGGCACCA-3’

CD: cluster of differentiation. General microglia markers: P2Y12: P2Y purinoceptor 12; CD68 or microsialin; CD45 or protein tyrosine phosphatase, receptor type, C (PTPRC). M1 phenotype: CD86 or T-lymphocyte activation antigen CD86; CD32 or low-affinity immunoglobulin gamma Fc region receptor II. M2 phenotype: CD206 or macrophage mannose receptor 1; CD163 or scavenger receptor cysteine-rich type 1 protein.