Table 2.

Transferase activity of EfPDT and DpNDT.

EfPDT specific activity (IU/mg)a,b				DpNDT specific activity (IU/mg)c
	dAdo	dGuo	dIno		dAdo	dCyd	dIno	dThd	dUrd
Ade	–	0.07	0.13	Ade	–	0.02	0.11	0.21	0.14
Gua	0.13	–	0.10	Cyt	0.02	–	0.04	0.04	0.06
Hyp	0.08	0.05	–	Hyp	0.02	0.01	–	0.72	0.67
				Thy	0.02	0.01	0.10	–	0.70
				Ura	0.07	0.02	0.11	0.73	–

^aReaction conditions: 5.375 μg of EfPDT in 40 μL at 50 ^◦C for 10 min, with 1 mM substrates in 50 mM MES buffer, pH 6.5 in a final volume of 40 μL

^bStandard deviation values <0.005 in all cases.

^c14.3 μg of DpNDT in 40 μL at 50 ^◦C for 20 min, with 10 mM substrates in 50 mM MES buffer, pH 6.5 in a final volume of 40 μL.