Table 2.
Concentrations of saxitoxin (μg·100 g−1) resulting from enzyme-linked immunosorbent assay (ELISA) analysis of tissues from common murres (Uria aalge) that suffered acute toxicity resulting in death or were euthanized at the conclusion of the median effective dose (ED50) dosing study. Individuals received either purified saxitoxin dihydrochloride (STX-diHCl) or an equivalent dose of a crude Alexandrium catenella extract (IC-8 B1). Abbreviations are as follows: BDL = below detection limits, DBNQ = detectable but not quantifiable, UGI = upper gastrointestinal tract, Sm Intestine = small intestine, Lg intestine = large intestine, NT = not tested. Dose is expressed as μg STX·kg−1 for STX-diHCl and μg STX-equivalents (eq)·kg−1 for the A. catenella extract. Tissue values are expressed as μg STX-eq·100 g−1.
| Bird ID | UA49 | UA54 | UA55 | UA46 | UA66 | UA48 | UA64 |
|---|---|---|---|---|---|---|---|
| Toxin type | STX-diHCl | STX-diHCl | STX-diHCl |
A. catenella Extract |
A. catenella Extract |
A. catenella Extract |
A. catenella Extract |
| Dose | 89 | 89 | 100.5 | 104.5 | 104.5 | 415.9 | 415.9 |
| Tissue | |||||||
| Serum | BDL | BDL | BDL | BDL | BDL | NT | NT |
| Muscle | BDL | BDL | BDL | BDL | BDL | BDL | BDL |
| Liver | BDL | BDL | DBNQ | BDL | BDL | 1.8 | BDL |
| UGI | 3.6 | 3.4 | 26.4 | 2.6 | 7.2 | 29.4 | 23.3 |
| Sm Intestine | 22.0 | 4.2 | 20.9 | 13.7 | 4.8 | 37.0 | 52.2 |
| Lg Intestine | 18.5 | 42.5 | 23.7 | 49.0 | 13.1 | 43.3 | 31.7 |
| Heart | BDL | BDL | 2.4 | BDL | BDL | 1.8 | BDL |
| Lung | BDL | BDL | 3.3 | BDL | BDL | 5.8 | 1.2 |
| Brain | BDL | BDL | BDL | BDL | BDL | BDL | BDL |
| Kidney | 0.7 | BDL | 2.0 | 1.7 | 0.6 | 4.2 | 1.5 |
| Spleen | BDL | BDL | BDL | BDL | BDL | 14.3 | BDL |
| Feces | 379.0 | 198.4 | NT | 66.5 | 179.6 | NT | NT |
| Regurgitant | 235.0 | 145.5 | NT | NT | NT | NT | NT |