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. 2025 Aug 20;57(9):2238–2249. doi: 10.1038/s41588-025-02300-4

Extended Data Fig. 5. Validation of myeloid-specific transcription factors (TFs), IRF4, MEF2B, and CEBPG, using external ENCODE ChIP-seq data.

Extended Data Fig. 5

a-c, Violin plots reflecting the empirical null distribution (n = 1,000) of the percentages of DMRs overlapping ChIP-seq peaks in each experiment, stratified by the SAT cell-types. Diamonds mark the observed overlapping proportions of the corresponding cell-type level hypo-methylated regions. *Indicates the statistical significance after multiple hypothesis correction (Bonferroni) on the empirical one-tailed P values for the over-representation of TF peaks. IRF4 in GM12878 cell line (empirical unadjusted P=0.001 for myeloid, mast, and lymphoid cells) (a); MEF2B in GM12878 cell line (empirical unadjusted P=0.001 for perivascular, myeloid, mast, and lymphoid cells) (b); and CEBPG in K562 cell line (empirical unadjusted P=0.001 for adipocytes, myeloid, and mast cells) (c). ChIP-seq, Chromatin immunoprecipitation sequencing; ASPC, adipose stem and progenitor cell; GM12878, lymphoblastoid cell line; and K562, chronic myelogenous leukemia cell line.