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HPLC analysis of the supernatant of a fermentation of P. griseofulvum NRRL 2300. (A) Control sample of penicillin G at a concentration of 0.1 mg/ml. The retention time for the penicillin G peak was 13.18 min. (B) Chromatogram of an ethyl acetate-extracted sample of that supernatant. (C) Chromatogram of the unprocessed supernatant. The extraction with ethyl acetate partially purifies and enriches the penicillin G in the sample.
