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. 2002 Mar;68(3):1211–1219. doi: 10.1128/AEM.68.3.1211-1219.2002

FIG. 3.

FIG. 3.

Presence of the genes pcbC and penDE in penicillin-producing strains and absence in nonproducing strains. EcoRI-digested genomic DNA from all the strains under study was electrophoresed (lanes 1 to 34), transferred to a nylon membrane, and hybridized with probe A (corresponding to the pcbC gene) and probe B (corresponding to the penDE gene). Lanes: 1 and 2, P. chrysogenum NRRL 1951 and AS-P-78; 3 and 4, P. nalgiovense NRRL 911 and 16a; 5 to 10, P. griseofulvum NRRL 2300, NRRL 2152, NRRL 989, NRRL 994, NRRL 991, and NRRL 992; 11 and 12, P. roqueforti CONT1 (isolated from blue cheese) and NRRL 849; 13, Paecilomyces variotii NRRL 1775; 14 to 17, P. camemberti NRRL 874, NRRL 877, NRRL 876 (ex-type of P. candidum), and CECT 2267; 18, P. commune INBCC300 (isolated from cecina); 19, P. chrysogenum AS-P-78; 20, P. nalgiovense 16a; 21 and 22, P. verrucosum NRRL 5573 and NRRL 5574; 23, P. brevicompactum NRRL 862; 24 and 25, P. commune NRRL 845 and CEC1 (isolated from cecina); 26, P. solitum NRRL 937; 27, P. crustosum NRRL 972; 28, P. expansum NRRL 976; 29, P. implicatum NRRL 2061; 30, P. hirsutum NRRL 2032; 31, P. aurantiogriseum NRRL 971; 32, P. viridicatum NRRL 963; 33, P. echinulatum NRRL 1151; 34, P. purpurogenum ATCC MYA-38.