TABLE 2.
Oligonucleotide sequences used for detection of enteric viruses
| Virus | Primer | Probe | Sequencea | Reference |
|---|---|---|---|---|
| Enterovirus | Pan-entero+(444-450) | CCTCCGGCCCCTGAATG | 34 | |
| Pan-entero−(621-638) | ACCGGATGGCCAATCCAA | 34 | ||
| TaqMan Probe | (FAM)CCGACTACTTTGGGTGTCCGTGTTTC (TAMRA) | This study | ||
| Norwalk virusa type G1 | COG1F | CTTAGACGCCATCATCATTYAC | K. Katayama et al., unpublished | |
| COG1R | CGYTGGATGCGNTTYCATGA | K. Katayama et al., unpublished | ||
| TaqMan Probe | (TET)AGATYGCGATCYCCTGTCCA(TAMRA) | K. Katayama et al., unpublished | ||
| Norwalk virus type G2 | COG2F | CARGARBCNATGTTYAGRTGGATGAG | K. Katayama et al., unpublished | |
| COG2R | TCGACGCCATCTTCATTCACA | K. Katayama et al., unpublished | ||
| TaqMan Probe | (FAM)TGGGAGGGCGATCGCAATCT(TAMRA) | K. Katayama et al., unpublished | ||
| HAV | HAV+ | CAGCACATCAGAAAGGTGAG | 42 | |
| HAV− | CTCCAGAATCATCTCCAAC | 42 |
a
Single-letter code: y stands for C or T; N, stands for A, C, G, or T; R stands for A or G; and B stands for B, T, or C. Abbreviations: FAM, 6-carboxy-fluorescein; TET, tetrachloro-6-carboxy-fluorescein; TAMRA, 6-carboxy-tetramethyl-rhodamine.