TABLE 5.
Recovery yields of poliovirus spiked in 1 liter of natural seawater by negatively charged membrane followed by centrifugal ultrafiltration
| Seawatera sampling place | Total coliforms (CFU/ml) | Fecal coliforms (CFU/100 ml) | Spiked amountb (PFU) | Poliovirus concn recovered (PFU/ml) | Vol of concentratec (ml) | % Recovery |
|---|---|---|---|---|---|---|
| Enoshima | 0 | 0 | 1,650 | 240 | 5 | 73 |
| Daiba | 3 | 25 | 1,650 | 225 | 5 | 68 |
| Kasai | 77 | 1,815 | 1,650 | 200 | 5 | 61 |
a
Enteroviruses were not detected by cell culture RT-PCR from 2 liters of each seawater sample.
b
Spiked in 1 liter of natural seawater.
c
Volumes of the concentrates were adjusted with Eagle's minimum medium.