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. Author manuscript; available in PMC: 2026 Jan 24.
Published in final edited form as: Cell Stem Cell. 2025 Aug 14;32(9):1421–1437.e6. doi: 10.1016/j.stem.2025.07.010

Figure 1: Extracellular matrix analysis reveals fibrotic and regenerative trajectories in bleomycin induced skin fibrosis.

Figure 1:

(A) Schematic of automated matrix analysis pipeline composed of 294 ultrastructural features. (B) H&E analysis of different timepoints following bleomycin injections. (C) Temporal progression of dermal matrix architecture following bleomycin treatment. Top; UMAPs of Picrosirius Red Staining Algorithm. Bottom; Representative PicroSirius Red staining of each timepoint. Fibrotic dermis exhibited progressive matrix aberration until Postoperative day 12 (POD 12), followed by resolution to baseline by POD 35. (D) Pseudotime analysis of matrix analysis. Left; UMAP plot of timepoints. Right: UMAP of Pseudotime analysis of matrix analysis colored by Pseudotime. (E) Kinetics of matrix progression using picrosirius red staining, identifying POD 12 and 21 as snapshots of active fibrosis and early remodeling, respectively. Scale bar (B) 250 μm. (C) 40 μm. n = 6 per timepoint unless otherwise stated.