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. Author manuscript; available in PMC: 2026 Jan 24.
Published in final edited form as: Cell Stem Cell. 2025 Aug 14;32(9):1421–1437.e6. doi: 10.1016/j.stem.2025.07.010

Figure 2: Single cell analysis of fibrotic and remodeling skin reveals fibroblast subcluster specific to the fibrotic phase.

Figure 2:

(A) Schematic of sequencing of baseline, fibrotic, and remodeling skin samples (B) Left; UMAP of overall cell phenotypes identified from single-cell transcriptomic profiling of skin. Right: UMAP of fibroblasts identified from single-cell transcriptomic profiling of skin. (C) Proportion analysis of fibroblast subtypes over time. (D) Gene analyses of top differentially expressed markers of all fibroblast clusters. Top; Fibroblast Cluster 0 Markers. Blue box shows expression of cluster defining markers. Bottom; Fibroblast Cluster 3 Markers. Purple box shows expression of cluster defining markers. (E) Gene ontology (GO) pathway and GO term analysis of cluster 0 (Top) and cluster 3 (Bottom). (F) Cell chat signaling of pathways enriched in Day 21 compared to Day 12 signaling including Apolipoprotein E (APOE) and Oncostatin (OSM) signaling (Top). Immunostaining of APOE and OSM in Day 21 skin (Bottom). Scale bar (F) 300 μm.