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. Author manuscript; available in PMC: 2026 Jan 24.
Published in final edited form as: Cell Stem Cell. 2025 Aug 14;32(9):1421–1437.e6. doi: 10.1016/j.stem.2025.07.010

Figure 4: Spatial analysis during fibrosis and repair in skin fibrosis reveals Cyp26b1 associated with fibrosis at a spatial level.

Figure 4:

(A) Schematic analysis of Visium and PhenoCycler analysis on different timepoints. (B) UMAP of spatial RNAseq clusters identified by Visium analysis. (C) Visualization of spatially indexed cell type probabilities for various cell phenotypes. (D) Violin plot of Cytochrome P450 26B1 (Cyp26b1), Family 2 Cyp26b1 and Yes Associated Protein 1 (Yap1) expression spatially by fibroblast subclusters. (E) Spatial plot of fibroblast subtype 1 at Day 12 (Top) and Day 21 (bottom). (F) Gene ontology pathway of fibroblast cluster 1 (Top) and spatial plot of Serpin family A member 1 (Serphinh1) at Day 12. (G) Identification of cellular niches associated with each fibroblast subtype. (H) Spatial plot of Cyp26b1 expression at Day 12 and Day 21. (I) UMAP of all cell types identified through PhenoCycler analysis. (J) Ridge plot of Collagen Type 1(Col1), Yap1, Cyp26b1, Piezo1, Piezo2 and Focal Adhesion Kinase (Fak) expression modules analysis across fibroblast subtypes.