Star Methods
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Mouse Monoclonal eFluor 450, CD45 | Thermo Fisher Scientific | Cat# MCD4528 |
| Anti-Mouse Monoclonal eFluor 450, Ter-119 | Thermo Fisher Scientific | Cat# 48-5921-82; |
| Anti-Mouse Monoclonal eFluor 450, CD31 | Biolegend | Cat# RM5228 |
| Anti-Mouse Monoclonal eFluor 450, Tie-2 | Thermo Fisher Scientific | Cat# 13-5987-82 |
| Anti-Mouse Monoclonal eFluor 450, CD326 | Thermo Fisher Scientific | Cat# 11-5791-82 |
| Anti-Mouse Monoclonal eFluor 450, CD324 | Thermo Fisher Scientific | Cat# 13-3249-82 |
| Anti-Mouse Monoclonal eFlor FITC | Thermo Fisher Scientific | Cat# MA5-17980 |
| Polyclonal CD82 | Thermo Fisher Scientific | Cat# PA5-13228 |
| Polyclonal TEM1/CD248 | Thermo Fisher Scientific | Cat# BS-2101R |
| Anti-Mouse CD55 eFlor PE | Thermo Fisher Scientific | Cat# 131803 |
| Anti-Rabbit Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-11008 |
| Anti-Rabbit Alexa Fluor 594 | Thermo Fisher Scientific | Cat# A-11037 |
| Anti-Rabbit Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A-21245 |
| Rabbit Anti-CD200 | Abcam | Cat# A-314662 |
| Rabbit Anti-CD82 | ThermoFisher Scientific | PA5-13228 |
| Rabbit Anti-CD248 | Bioss | BS-2101R |
| Rabbit Anti-APOE | Proteintech | 18254-1-AP |
| Rabbit Anti-OSM | Novus | AF-495-SP |
| Rabbit Anti-CYP26B1 | ThermoFisher Scientific | 21555-1-AP |
| Rabbit Anti-RARRES2 | Proteintech | 10216-1-AP |
| Rabbit Anti-COLLAGEN1A1 | ThermoFisher Scientific | PA5-29569 |
| Chemicals, peptides, and recombinant proteins | ||
| Bleomycin | Millipore Sigma | Cat# 1076308 |
| Talarozole | MedChemExpress | Cat# R115866 |
| Fluoromount-G with DAPI | Thermo Fisher Scientific | Cat# 00-4959-52 |
| Permount | Fisher Chemicals | Cat# SP15 |
| Collagenase II/IV | ThermoScientifc | Cat# 17101015, 17104019 |
| Biogenex Laboratories Power Block | Fisher Scientific | Cat# NC9495720 |
| Trypsin antigen retrieval solution | Abcam | Cat# ab970 |
| Ethanol | GoldShield | Cat# 64175 |
| Xylene | Sigma | Cat# 534056 |
| Triton-X 100 | Sigma | Cat# X100 |
| Phosphate Buffered Saline | Sigma | Cat# P5368 |
| DAPI | BioLegend | Cat# 422801 |
| Hematoxylin | Sigma | Cat# H3136 |
| Eosin | Sigma | Cat# HT1101128 |
| Picro Sirius Red stain kit | Abcam | Cat# ab150681 |
| Mouse ELISA | MyBioSource | Cat# MBS706971 |
| Critical commercial assays | ||
| 10x Chromium Single Cell platform | 10X Genomics | Single Cell 3’ v3 |
| 10x Chromium Single Nuclei platform | 10X Genomics | Single Cell ATAC v2 |
| Visium Tissue Optimization Slide and Reagent Kit | 10X Genomics | Visium Tissue Optimization Slide and Reagent Kit |
| Agilent Bioanalyzer | Agilent | RRID:SCR_018043 |
| Experimental models: Organisms/strains | ||
| C57/BL/6J | The Jackson Laboratory | Stock No. 000664 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO: GSE278069 GSE278169 GSE277889 |
| Software and algorithms | ||
| ImageJ | National Institutes of Health | RRID:SCR_003070 |
| MATLAB | MathWorks | RRID:SCR_001622 |
| Rstudio | RStudio | RRID:SCR_000432 |
| Other | ||
| Tegaderm dressings | 3M | Cat# 1624W |