Figure 1. Culture of muscle layer-derived cells prepared from embryonic hindgut.

(A) Culture of muscle layer-derived cells prepared from embryonic hindgut with FBS free-media and substrates. (B) Long-term time-lapse imaging after seeding on Matrigel with Neurobasal media. The images show the ability of these cells to self-assemble at 0, 20, 40, 60, 80, and 100 hours taken from Figure 1—video 1. Scale bars: 100 μm (A, B).

Figure 1—figure supplement 1. Dissection and layers of the E15 chicken embryonic hindgut.

(A) Chicken embryonic hindgut at E15. It was dissected from the bottom of the cecum to the front of the cloaca (white lines). (B) Three layers of E15 hindgut: serosa, muscle layer, intestinal epithelium. Remak’s ganglion was also removed. Scale bars: 1 mm in A, B.

Figure 1—video 1. Long-term time-lapse imaging after seeding.

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A time lapse was taken every 2 hr for a total of 100 hr. This video corresponds to Figure 1B. Scale bar: 200 μm.