Figure 2. Spheroids formed in Neurobasal medium and Matrigel exhibited reiterated contractions.
(A) Clusters/spheroids at days 3, 5, and 7 exhibited reiterative contractions. Graphs show normalized contraction intensities visualized using the Time Measurement function. Arrowheads indicate contraction peaks defined by a peak prominence > 0.25 and a peak width ≤ 10 seconds. (B) Contraction intervals in clusters/spheroids from day 3 to day 7. Each dot represents a single contraction interval. Median values: day 3, 13.3; day 5, 15.4; day 7, 19.6. Sample sizes: day 3, n=35, peak count=646; day 5, n=48, peak count=748; day 7, n=21, peak count=250. Scale bars: 50 µm (A).
Figure 2—source data 1. Time lapse data for Figure 2 and Figure 2—video 1.
elife-97860-fig2-data1.xlsx (424.2KB, xlsx)
Figure 2—video 1. Periodic contractions of the cluster/spheroid on days 3, 5, and 7.
Download video file (4.6MB, mp4)
Time-lapse images were taken with 700 ms intervals for 5 min. This video corresponds to Figure 2A. Scale bars: 50 µm.