Figure 4. Ca²⁺ imaging of the gut contractile organoid revealed intercellular synchronization.

(A) RCAS-GCaMP6s-P2A-mRuby3 plasmid. (B) Ca²⁺ imaging of gut contractile organoid during relaxation and contraction. Ca²⁺ dynamics (green) and normalized values of contraction (gray) of gut contractile organoid. (C) Simultaneous measurement of intercellular Ca²⁺ dynamics between ICC-ICC, SMC-SMC, or ICC-SMC. Three or two ROIs in Ca²⁺ signal-positive cells were set in a single organoid. Graphs show Ca²⁺ dynamics in the ROIs. Magnified view shows that a peak of Ca²⁺ signal in ICC (green) preceded that in SMC (magenta). (D) Peak latency in ICC/SMC. Median value = 0.7; Sample size: n = 14, peak count  = 104. Scale bars: 50 µm (B).

Figure 4—video 1. Ca²⁺ dynamics in day 7 gut contractile organoid are concomitant with its contractions.

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Time-lapse images were obtained with 700 ms intervals for 5 min. This video corresponds to Figure 4. Scale bar: 50 µm.