Figure 6. The organoidal contraction is important for Ca²⁺ dynamics in ICCs.

GCaMP6s-expressing organoids were cultured with Blebbistatin. (A) Organoidal contractions were extinguished at 10 µM. (B) Contraction intervals before and after Blebbistatin, and upon washout. Median values: Before, 33.46; Blebbistatin 5  µM, 12.86; 10 µM, N/A; Washout, 23.95. Statistical significance was assessed using Welch’s t-test. ***p<0.001, *p<0.05. Sample size: n=3. (C–E) Comparison of Ca²⁺ dynamics in SMC-SMC, ICC-ICC, and ICC-SMC. Three or two ROIs were assessed before and after administrations of 10 µM Blebbistatin. (F) Ca²⁺ transients in a single ICC at 0 μM (before), 5 μM, and 10 μM. Scale bar: 50 µm (B).

Figure 6—video 1. Ca²⁺ dynamics in day 7 gut contractile organoid with Blebbistatin.

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Time-lapse images were obtained with 700 ms intervals for 5 min. This video corresponds to Figure 6. Scale bars: 50 µm.