Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Gallus gallus) | Embryonic hindgut | Yamagishi poultry farms (Wakayama, Japan) | Freshly isolated from Gallus gallus | |
| Biological sample (Gallus gallus) | Embryonic hindgut | Takeuchi Farm (Nara, Japan). | Freshly isolated from Gallus gallus | |
| Antibody | anti-c-Kit (Rabbit polyclonal) | Sigma Aldrich Japan; Yagasaki et al., 2022 | IF(1:300) | |
| Antibody | anti-Tuj-1 (Mouse monoclonal) | RSD | MAB1195 RRID:AB_357520 |
IF(1:500) |
| Antibody | anti-αSMA (Mouse monoclonal) |
Sigma-Aldrich | A5228 RRID:AB_262054 |
IF(1:400) |
| Antibody | anti-Desmin (Mouse monoclonal) | Novus Biologicals | NBP1-97707 RRID:AB_3243420 |
IF(1:400) |
| Antibody | anti-chicken N-cadherin (Rat monoclonal) | TAKARA | M110 | IF(1:200) |
| Cell line (Gallus gallus) | DF-1 | ATCC | CRL-3586 RRID:CVCL_0570 |
fibroblast cell line isolated from chicken embryo |
| Transfected construct | GCaMP6s-P2A-mRuby3 | Addgene | 112007 RRID:Addgene_112007 |
|
| Chemical compound, drug | Carbenoxolone | nacalai tesque | 32775–51 | |
| Chemical compound, drug | 18beta-Glycyrrhetinic acid | abcam | ab142579 | |
| Chemical compound, drug | (-)-Blebbistatin | FUJIFILM Wako | 021–17041 | |
| Chemical compound, drug | Nifedipine | FUJIFILM Wako | 141–05783 | |
| Software, algorithm | MATLAB | MathWorks | findpeaks | |
| Other | Matrigel | Corning | 354248 | |
| Other | Neurobasal medium | Gibco | 21103–049 | |
| Other | 50× B-27 supplement | Gibco | 17504044 |