Figure 2. Solution and TEM characterization of βCDRhuA and azoRhuA co-assembly.

(A) DLS measurements showing the time-dependent evolution of βCDRhuA-azoRhuA co-assemblies.
(B) Yield of βCDRhuA and azoRhuA co-assembly under different [βCDRhuA]:[azoRhuA ] ratios at constant total protein concentration, as monitored by turbidity measurements (at 425 nm) after 3 h of mixing.
(C) Negative-stain TEM images of βCDRhuA-azoRhuA nanotubes at different magnifications. Power spectrum of one of the nanotubes is shown in the inset in the right panel.
(D) Distribution of βCDRhuA-azoRhuA nanotubes with different diameters, determined from negative-stain TEM images.